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Literature summary extracted from

  • Goto, M.; Shinmura, K.; Matsushima, Y.; Ishino, K.; Yamada, H.; Totsuka, Y.; Matsuda, T.; Nakagama, H.; Sugimura, H.
    Human DNA glycosylase enzyme TDG repairs thymine mispaired with exocyclic etheno-DNA adducts (2014), Free Radic. Biol. Med., 76, 136-146.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.29 expressed in Escherichia coli Bl21-CodonPlus (DE3)-RP cells Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.29 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.29 glutathione Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.29 thymine-mismatched double-stranded DNA + H2O the enzyme is capable of removing thymine that has mispaired with 3,N4-ethenocytosine, butanone-ethenocytosine, butanone-ethenoguanine, heptanone-ethenocytosine, or heptanone-ethenoguanine in vitro Homo sapiens thymine + double-stranded DNA with abasic site
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.29 TDG
-
 Homo sapiens
3.2.2.29 thymine DNA glycosylase
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
3.2.2.29 malfunction enzyme-knockdown cells exhibit higher resistance to cell death caused by the induction of etheno-DNA adducts, lower repair activityfor 3,N4-ethenocytosine, and a modest acceleration of mutations caused by 3,N4-ethenocytosine,compared with the rate in control cells Homo sapiens
3.2.2.29 physiological function the enzyme shows repair activities toward etheno-DNA adducts Homo sapiens